Structure of the human UFC1 protein in complex with the UBA5 C-terminal UFC1-binding motif.

Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5

The default ambiguity codes are applicable.

3D F1-13C/15N-filtered NOESY-[13C,1H]-HSQC

3D F1-13C/15N-filtered NOESY-[15N,1H]-SOFAST-HMQC

3D F1-13C/15N-filtered NOESY-[13C,1H]-SOFAST-HMQC (aro)

3D NOESY-[13C,1H]-HSQC (aliphatic region)

International journal of molecular sciences
A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1.

Ubiquitin-activating enzyme E1 domain-containing protein 1

1.0 mM [U-100% 13C; U-100% 15N] Ubiquitin-fold modifier-conjugating enzyme 1,
1.0 mM Ubiquitin-like modifier-activating enzyme 5, 50 mM TRIS,
100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor,
0.15 mM DSS, 95% H2O/5% D2O.

1.2 mM Ubiquitin-fold modifier-conjugating enzyme 1,
0.3 mM [U-100% 13C; U-100% 15N] Ubiquitin-like modifier-activating enzyme 5,
50 mM TRIS, 100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor,
0.15 mM DSS, 95% H2O/5% D2O.

Ubiquitin-fold modifier-conjugating enzyme 1

Ubiquitin-like modifier-activating enzyme 5