Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues

A fragment-based approach applied to a highly flexible target: Insights and challenges towards the inhibition of HSP70 isoforms.

NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381

BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1

HEAT SHOCK 70 KDA PROTEIN 8, LIPOPOLYSACCHARIDE-ASSOCIATED PROTEIN 1, LAP-1, LPS-ASSOCIATED PROTEIN 1

16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL

HEAT SHOCK COGNATE 71 KDA PROTEIN (E.C.3.6.3.51), BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1

HEAT SHOCK PROTEIN, HSP70, HSP72, HSC70, ATPASE, BAG1, CHAPERONE, FRAGMENT