Crystal Structure of monoclonal Fab F22-4 specific for Shigella flexneri 2a O-Ag

Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody

Diamond(111), Ge(220), sagitally focusing Ge(220), multilayer

24% PEG 2000 MME, 190mM MgCl2, 50mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Fab F22-4 in complex with a Shigella flexneri 2a O-Ag decasaccharide

Fab F22-4 in complex with a Shigella flexneri 2a O-Ag pentadecasaccharide

THE SEQUENCE DATABASE FOR CHAINS A,C RESIDUES 1-107 IS
CAH04481.2, AND FOR CHAINS B,D RESIDUES 1-113 IS
CAH04480.1.
RESIDUE NUMBERING OF ANTIBODY VARIABLE REGIONS FOLLOWS THE
KABAT CONVENTION (E.A. KABAT, T.T. WU, H.M. PERRY, K.S.
GOTTESMAN, C. FOELLER. SEQUENCES OF PROTEINS OF
IMMUNOLOGICAL INTEREST, 5TH ED. (1991). NIH PUBLICATION NO.
91-3242, NATIONAL INSTITUTES OF HEALTH, BETHESDA, MD).
DISCREPANCIES BETWEEN THE DATABASE SEQUENCE CAH04481.2 AND
THIS PDB ENTRY OCCUR AT POSITIONS ASP 1, ILE 2, ALA 7, ALA
8, AND PHE 9 (CHAINS A,C), AND BETWEEN DATABASE SEQUENCE
CAH04480.1 AND THIS ENTRY AT POSITION VAL 4 (CHAINS B,D).
THESE N-TERMINAL SEQUENCE DISCREPANCIES ARE DUE TO THE
PRIMERS USED FOR NUCLEOTIDE SEQUENCE DETERMINATION.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
VL/VH dimer: pdb entry 1ejo residues 2002L-2109L; pdb entry 1aj4 residues 1H-99H; CL/CH dimer: pdb entry 1f11 residues 108A-211A and 114B-227B.

Fab F22-4 light chain, Fab F22-4 heavy chain

Antibody, O-Antigen, LPS, Shigella flexneri, IMMUNE SYSTEM