Structure of lectin from the sea mussel Crenomytilus grayanus

Structure of a lectin from the sea mussel Crenomytilus grayanus (CGL).

The synthetic gene was cloned in the Gateway (R) system with the N-TERMINAL HIS6-TAG

Non-optimized sequence synthesized by BioiBasic

0.1 M HEPES pH 7.0, 18% (w/v) PEG4000, 0.1 M SODIUM ACETATE, 0.1 M LITHIUM SUFFATE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Model was an electron density calculated for the assembly of 39 homologous structures

Monomer confirmed by gel filtration and X-ray analysis

lectin, sea vertebrate, recombinant, trefoil, SUGAR BINDING PROTEIN