Human Dis3L2 in complex with hairpin D-U7

A shape-shifting nuclease unravels structured RNA.

The model of Homo Sapiens Dis3L2 was used as a starting reference for the protein and the nucleic acid was used from PDB 4PMW. The CSDs were repositioned to fit into the repositioned density via rigid body fit and the linker was rebuilt manually. Base pairing restraints were used for the base of the RNA hairpin for which density was present. All manual building was done in Coot. The model was further refined using PHENIX.

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

Wild-type HsDis3L2 in complex with hairpinD-U7

The full sequence of the hairpin in the sample is: CGGCGUUUCGACGCCGUUUUUUU. Some of the hairpin, including the tetraloop, was not resolved in the structure.

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Human Dis3L2 in complex with hairpinD-U7

The complex eluted as a homogeneous, Gaussian peak, shifted to a smaller elution volume as compared to the protein or RNA alone and the 260 nm absorption was greater than that of the 280 indicating the presence of RNA in that peak. The structure is highly similar to that of the mouse Dis3L2 (PDB: 4PMW)

3'-5' exonuclease, ds-RNA bound exonuclease, human exonuclease, RNA BINDING PROTEIN-RNA complex