Crystal structure of E. coli aspartate aminotransferase mutant VFIT in the ligand-free form at 303 K

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Computational remodeling of an enzyme conformational landscape for altered substrate selectivity

HEPES, ammonium sulfate, PEG 400, maleate

Natural Sciences and Engineering Research Council (NSERC, Canada)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

chain 'A' and (resid 0 through 32 )

chain 'A' and (resid 33 through 59 )

chain 'A' and (resid 60 through 130 )

chain 'A' and (resid 131 through 300 )

chain 'A' and (resid 301 through 331 )

chain 'A' and (resid 332 through 396 )

chain 'B' and (resid 0 through 32 )

chain 'B' and (resid 33 through 59 )

chain 'B' and (resid 60 through 101 )

chain 'B' and (resid 102 through 168 )

chain 'B' and (resid 169 through 222 )

chain 'B' and (resid 223 through 300 )

chain 'B' and (resid 301 through 331 )

chain 'B' and (resid 332 through 365 )

chain 'B' and (resid 366 through 396 )

Enzyme, Designed Protein, Mutant Protein, TRANSFERASE