Crystal Structure of beta-methylaspartase from Clostridium tetanomorphum. Apo-structure.

The structure of 3-methylaspartase from Clostridium tetanomorphum functions via the common enolase chemical step.

Overexpression, purification, crystallization and data collection of 3-methylaspartase from Clostridium tetanomorphum

Tris-HCl, NaCl, PEG6000, ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K

TLS-option in REFMAC5 with 3 TLS groups for protein chains and water molecules. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
BABINET model with mask parameters for mask calculation