Crystal structure of Acetyl xylan esterase (TM0077) from THERMOTOGA MARITIMA at 2.50 A resolution (native apo structure)

Joint Center for Structural Genomics (JCSG)

Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima.

0.2000M CaAcetate, 20.0000% PEG-3350, No Buffer pH 7.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystal structure of acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution (selenomethionine substituted structure, monoclinic crystal form)

Crystal structure of acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.40 A resolution (PMSF inhibitor complex structure, orthorhombic crystal form)

Crystal structure of acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.12 A resolution (paraoxon inhibitor complex structure, orthorhombic crystal form)

SEQUENCE: THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHH FOLLOWED BY THE TARGET SEQUENCE.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2. CALCIUM (CA), CHLORIDE (CL) AND ACETATE (ACT) IONS FROM CRYSTALLIZATION CONDITIONS, AND ETHYLENE GLYCOL (EDO) MOLECULES FROM CRYO CONDITION ARE MODELLED.

AUTHORS STATE THAT SIZE EXCLUSION CHROMATOGRAPHY WITH STATIC LIGHT SCATTERING SUPPORTS THE ASSIGNMENT OF A HEXAMER AS A SIGNIFICANT OLIGOMERIZATION STATE IN SOLUTION.

Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI-2, AXE1, HYDROLASE