Discovery of the Highly Potent PI3K/mTOR Dual Inhibitor PF-04691502 through Structure Based Drug Design

Discovery of the Highly Potent PI3K/mTOR Dual Inhibitor PF-04979064 through Structure-Based Drug Design.

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform

PtdIns-3-kinase subunit gamma, PI3-kinase subunit gamma, PI3K-gamma, Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, PtdIns-3-kinase subunit p110-gamma, p120-PI3K

Protein] : [Compound] = 1:10

Hanging drop: 2.5ul protein + 2.5ul well @ 13 C

Well solution: 0.1M Bis-Tris propane, pH8.2, 0.2M (NH4)2SO4, 16-18% (w/v) PEG3350, 10% (v/v) Ethylene Glycol and 10mM TCEP, pH7.0

, VAPOR DIFFUSION, HANGING DROP, temperature 286K

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform (E.C.2.7.1.153)/Inhibitor PF-04691502 complex

phosphoinositide kinase, inhibition, TRANSFERASE-TRANSFERASE INHIBITOR complex