Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:2:2 complex (core complex)

Architectural principles for Hfq/Crc-mediated regulation of gene expression

Assembly of Hfq-Crc-amiE with a 2:2:2 stoichiometry

Images were collected in movie-counting mode, 75 frames per movie stack, 60s exposure per movie

RNA (5'-R(*AP*AP*AP*AP*AP*UP*AP*AP*CP*AP*AP*CP*AP*AP*GP*AP*GP*G)-3')

Exodeoxyribonuclease,Exodeoxyribonuclease III

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Gel filtration and Multi Angle Light Scattering

Catabolite repression control protein, RNA-binding protein Hfq/RNA Complex

Hfq, Crc, amiE, Carbon catabolite repression, RNA-protein interaction, RNA-mediated gene regulation, RNA BINDING PROTEIN, RNA BINDING PROTEIN-RNA-Hydrolase complex