HcgC from Methanococcus maripaludis cocrystallized with SAH and pyridinol

(3~{E})-3-[(~{E})-3-oxidanylprop-2-enoyl]iminopropanoic acid

A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC.

The drop consists of 1 ul of enzyme solution containing 5 mg/ml HcgC, 2 mM pyridinol and 2 mM SAH mixed with 1 ul of the reservoir solution : 40% v/v PEG 400, 100 mM Tris/HCl pH 8.5 and 200 mM LiSO4

methyltransferases, biosynthesis, protein structures, enzyme catalysis, mutagenesis, [Fe]-hydrogenase, pyridinol, Hmd, Transferase