Structure of effector binding domain of central glycolytic gene regulator (CggR) from B. subtilis

Midwest Center for Structural Genomics (MCSG)

Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.

Rosenbaum-Rock high-resolution double-crystal, Si 111

Reservoir: 16% PEG3350, 0.1M MES pH 6.5, 0.1M MgCl2, 0.1M 6-aminohexanoic acid. Protein: 26.2mg/ml. Drops: 1+1 microliter, VAPOR DIFFUSION, HANGING DROP, temperature 298K

BIOMOLECULE: 1
THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT
WHICH CONSISTS OF 2 CHAIN(S). SEE REMARK 350 FOR
INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).
AUTHORS STATE THAT THE DIMER PRESENT IN THE ASYMMETRIC
UNIT IS PRESUMABLY THE BIOLOGICAL RELEVANT UNIT

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

dimer in asymmetric unit is presumably biological relevant unit

alpha/beta/alpha sandwich, Rossmann-like fold, Structural Genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG, TRANSCRIPTION