A non-invasive GFP-based biosensor for mercury ions

{2-[(1R,2R)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake.

Local complexity of amino acid interactions in a protein core.

Crystal structure of the Aequorea victoria green fluorescent protein.

100mM Pipes, 30% PEG 8000, 200mM Na-acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

A single mutation converts the green fluorescent protein into a highly specific and sensitive mercury (II) biosensor

AUTHORS STATE THAT RESIDUE 64 IS A LEUCINE. RESIDUE 65 HAS BEEN MUTATED FROM SER TO THR AND RESIDUE 80 IS AN ARG TO GLN MUTATION. RESIDUE 80 IS LISTED AS A GLN IN THE DATABASE REFERENCE BUT IS AN ARG ACCORDING TO ROUWENDAL ET AL., 1997 PLANT MOL. BIOL. 33, 989-999. RESIDUES THR 65, TYR 66, GLY 67 CONSTITUTE THE CHROMOPHORE CRO

First 6 residues and the last c-terminal residues could not be modeled into the density. These residues were therefore omitted in the final model