Cryo-EM structure of the Sulfolobus acidocaldarius RNA polymerase at 2.88 A

Structural basis of RNA polymerase inhibition by viral and host factors.

CTF aplitude was further corrected on selected particles after 3D recostruction to correct the 30 degrees tilt used during data collection

Movie stacks were aligned and summed using MotionCor2

Images were collected in movie-mode for a total of 40 frames in super-resolution mode and with a tilt of 30 degrees

The grid was coated with graphene oxide prior to use

The sample was crosslinked with bis(sulfosuccinimidyl)suberate (BS3)

DNA-directed RNA polymerase subunit RPO1

DNA-directed RNA polymerase subunit RPO2

DNA-directed RNA polymerase subunit RPO3

DNA-directed RNA polymerase subunit RPO7

DNA-directed RNA polymerase subunit RPO4

DNA-directed RNA polymerase subunit RPO8

DNA-directed RNA polymerase subunit RPO5

DNA-directed RNA polymerase subunit RPO6

DNA-directed RNA polymerase subunit RPO11

DNA-directed RNA polymerase subunit RPO10

DNA-directed RNA polymerase subunit RPO12

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

Cryo-EM structure of the archaeal RNA polymerase inhibited by RIP

Cryo-EM structure of the archaeal RNA polymerase inhibited by TFS4

monomeric in solution (by size exclusion chromatography) and on micrographs (by both negative staining and cryo-EM)
RNA polymerase, transcription, Archaea, Crenarchaea