X-Ray crystal structure of a nucleosome core particle with its DNA site-specifically crosslinked to the histone octamer

Site-Specific Disulfide Crosslinked Nucleosomes with Enhanced Stability.

Sample was mixed in a 1:1 ratio with 10 mM Na-cacodylate, pH 6.0, 130-180 mM MnCl2, 100-160 mM KCl and equilibrated against a 1:4 dilution of the same solution

constructs were all sequenced and the expressed protein MW was confirmed by mass spec: 30268543:42-452 Expression vector pET3-H3 H3 gene for Xenopus laevis-like histone H3 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA 30268542|emb|CAD89678.1| Xenopus laevis-like histone H2B [Expression vector pET3-H2B] MAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFER IAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Histone H3.2, Histone H4, Histone H2A, Histone H2B 1.1/DNA Complex

Nucleosome Core Particle, Histones, Disulfide, Convertible Nucleotide, DNA, DNA binding protein

binding site for Di-peptide CYS E 40 and G47 J 70