Complex of the Yersinia enterocolitica Type III secretion export gate YscV with substrate:chaperone complex YscX:YscY

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Direct interaction of a chaperone-bound type III secretion substrate with the export gate.

PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)

Protein Concentration: 3-5 mg/ml;

Protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 5 mM TCEP;

Reservoir Solution: 0.1 M Hepes pH 6.8-8.0, 1 % PEG 2'000 MME, 0.8-1.2 M sodium succinate;

Drop Ratio: 0.33 uL Reservoir + 0.66 uL Protein

Complex confirmed via co-expression, pull-down and gel filtration.
Type III Secretion System, T3SS, SctV, SctXY, PROTEIN TRANSPORT
(chain "AA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "BA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "CA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 704))
(chain "DA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 466 through 467 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "EA" and (resid 361 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "FA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "GA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "HA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "IA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "JA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "KA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "LA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 653 or resid 658 through 689 or resid 691 through 704))
(chain "MA" and (resid 361 through 387 or resid 390 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 657 through 689 or resid 691 through 704))
(chain "NA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "OA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "PA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "QA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "RA" and (resid 361 through 387 or resid 390 through 418 or resid 424 through 436 or resid 453 or resid 456 or resid 474 through 486 or resid 490 through 649 or resid 653 or resid 658 through 689 or resid 691 through 704))
(chain "AB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "BB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "CB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "DB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "EB" and (resid 52 through 89 or resid 94 through 122))
(chain "FB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "GB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "HB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "IB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "JB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "KB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "LB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "MB" and (resid 52 through 62 or resid 75 through 122))
(chain "NB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "OB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "PB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "QB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "RB" and (resid 52 through 62 or resid 75 through 89 or resid 94 through 122))
(chain "AC" and (resid 8 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "BC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "CC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "DC" and (resid 8 through 41 or resid 50 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "EC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 107))
(chain "FC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "GC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "HC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "IC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 107))
(chain "JC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "LC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "MC" and (resid 8 through 41 or resid 50 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "NC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "OC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "PC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "QC" and (resid 8 through 41 or resid 50 through 73 or resid 80 through 91 or resid 95 through 107))
(chain "RC" and (resid 8 through 41 or resid 50 through 58 or resid 62 through 73 or resid 80 through 91 or resid 95 through 107))