Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome

Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.

15 mM HEPES pH 7.5
100 mM NaCL
0.5 mM DTT
1% Glycerol

Ternary complex of COP9 signalosome (CSN) and neddylated Cullin-Ring E3 ligase CRL (cullin-2 with Rbx1, Elongin-B, Elongin-C and VHL)

von Hippel-Lindau disease tumor suppressor

Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH

Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TRIP-15

Signalosome subunit 3,JAB1-containing signalosome subunit 3

Signalosome subunit 4,JAB1-containing signalosome subunit 4

Signalosome subunit 5,Jun activation domain-binding protein 1

Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP

Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8

Signalosome subunit 7b,JAB1-containing signalosome subunit 7b

EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2

EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1

Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8

COP9 signalosome complex subunit 1, COP9 signalosome complex subunit 2, COP9 signalosome complex subunit 3, COP9 signalosome complex subunit 4, COP9 signalosome complex subunit 5 (E.C.3.4.-.-), COP9 signalosome complex subunit 6, COP9 signalosome complex subunit 8, COP9 signalosome complex subunit 7b, von Hippel-Lindau disease tumor suppressor, Elongin-B, Elongin-C, Cullin-2, E3 ubiquitin-protein ligase RBX1, NEDD8
Cullin-Ring E3 Ligase COP9 signalosome neddylation, LIGASE