Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome

Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.

15 mM HEPES pH 7.5
100 mM NaCl
0.5 mM DTT
1% Glycerol

CNS (COP9 Signalosome) with CSN5/CSN6 the catalytic heterodimer complexed with CUL2 (CUL2, ElonginB, ElongingC, Rbx1 and VDL)

Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH

Signalosome subunit 4,JAB1-containing signalosome subunit 4

Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8

EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2

EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1

Signalosome subunit 3,JAB1-containing signalosome subunit 3

Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TRIP-15

COP9 signalosome complex subunit 1, COP9 signalosome complex subunit 4, COP9 signalosome complex subunit 8, Elongin-B, Elongin-C, RBX1 E3 ubiquitin-protein ligase, COP9 signalosome complex subunit 3, CSN7B_HUMAN, Cullin-2, COP9 signalosome complex subunit 2
Cullin-Ring E3 Ligases (CRLs) COP9 signalosome (CSN) deneddylation VHL tumour suppressor substrate receptor, LIGASE