CryoEM structure of human Arp2/3 complex with bound NPFs

Cryo-EM structure of NPF-bound human Arp2/3 complex and activation mechanism.

Many peptide bonds are too short or too long

Cryo-EM structure of human Arp2/3 complex with bound NPFs

Data collected in super resolution mode. Illuminated area of 1.01um. Nominal Dose of 40a/A^2 and a dose rate of 4.87 e-/s/pixel. 2 or 5 exposures per hole by image shift.

Grids glow discharged with easiGLOW glow discharger at 0.3 mBar, 25mA for 1 minute

Grids manually blotted for 3 seconds with Whatman 41 filter paper and manually plunged on Leica EM CPC manual plunger.

Actin-related protein 2/3 complex subunit 1B

Actin-related protein 2/3 complex subunit 2

Actin-related protein 2/3 complex subunit 3

Actin-related protein 2/3 complex subunit 4

Actin-related protein 2/3 complex subunit 5

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)

Actin-related protein 3, Actin-related protein 2, Actin-related protein 2/3 complex subunit 1B, Actin-related protein 2/3 complex subunit 2, Actin-related protein 2/3 complex subunit 3, Actin-related protein 2/3 complex subunit 4, Actin-related protein 2/3 complex subunit 5, Neural Wiskott-Aldrich syndrome protein
actin, ATPase, actin related protein, arp, cytoskeleton, Arp2-3 complex, actin nucleation, actin branching, CONTRACTILE PROTEIN