Crystal Structure of PTE mutant H254G/H257W/L303T/M317L/I106C/F132I/L271I/K185R/I274N/A80V/R67H

Enzymes for the homeland defense: optimizing phosphotriesterase for the hydrolysis of organophosphate nerve agents.

Si(111), Side scattering bent cube-root I-beam single crystal, asymmetric cut 4.965 degs

2 uL 7.8 mg/mL protein + 2 uL seeding solution (24% PEG5000 MME, 4% dioxane, 1.0 mM cobalt chloride, 100 mM diethylphosphate, 0.1 M imidazole, pH 7.0) over 500 uL precipitating agent (22% PEG5000 MME), VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystal Structure of PTE mutant H254Q/H257F/K185R/I274N

Crystal Structure of PTE mutant H254G/H257W/L303T/K185R/I274N/A80V

Crystal Structure of PTE mutant K185R/I274N

Crystal Structure of PTE mutant H254G/H257W/L303T/K185R/I274N/A80V/S61T

Crystal Structure of PTE mutant H254G/H257W/L303T/K185R/I274N/A80V/S61T with cyclohexyl methylphosphonate inhibitor

Crystal Structure of PTE mutant H254G/H257W/L303T/M317L/I106C/F132I/L271I/K185R/I274N/A80V/R67H with cyclohexyl methylphosphonate inhibitor

metalloenzyme, TIM barrel, nerve agents, phosphotriesterase, HYDROLASE