The P-Loop K to A mutation of C. therm Vps1 GTPase-BSE

PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.

SI 111. ROSENBAUM-ROCK DOUBLE -CRYSTAL MONOCHROMATOR: LIQUID NITROGEN COOLED; SAGITALLY FOCUSING 2ND CRYSTAL, ROSENBAUM-ROCK VERTICAL FOCUSING MIRROR

Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

91 mM HEPES pH 8.1
16.7 % PEG 3,350
182 mM Sodium Acetate
3% PROPAN-2-OL

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Wild type protein (not the P-Loop Lysine to Alanine mutation herein reported) dimerizes in the presence of the transition state analog GDP.AlFx

GTPase, Dynamin, Dynamin Superfamily, BSE, GED, GCP, P-Loop, HYDROLASE