Structural and biochemical evidence for a boat-like transition state in beta-mannosidases

STRUCTURAL AND BIOCHEMICAL EVIDENCE FOR A BOAT-LIKE TRANSITION STATE IN BETA- MANNOSIDASES

STRUCTURE OF A BETA-MANNOSIDASE FROM BACTEROIDES THETAIOTAOMICRON

ENGINEERED RESIDUE IN CHAIN A, TRP 645 TO ALA ENGINEERED RESIDUE IN CHAIN B, TRP 645 TO ALA
THERE IS AN ENGINEERED MUTATION IN THE SEQUENCE, W645A.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 26-27, 318 AND 865-871 ARE DISORDERED IN CHAIN A, AND RESIDUES 26-27 AND 868-871 ARE DISORDERED IN CHAIN B.

LINEAR FREE ENERGY RELATIONSHIP, TRANSITION STATE MIMIC, MANNOSIDASE, GLYCOSIDE HYDROLASE, HYDROLASE