Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP

The Structural Basis for CAP Binding by Influenza Virus Polymerase Subunit Pb2.

RNA-DIRECTED RNA POLYMERASE SUBUNIT P3, PB2 CAP-BINDING DOMAIN

1 MICROLITRE OF PROTEIN SOLUTION AT 11 MG PER ML IN 50 MM TRIS-HCL (PH 8.0), 200 MM NACL, 2 MM DTT, 5 MM M7GTP WITH AN EQUAL VOLUME OF A SOLUTION CONTAINING 0.1 M CITRIC ACID PH 4.6, 1.6-1.8 M SODIUM FORMATE.

C-TERMINAL DOMAIN OF INFLUENZA A VIRUS POLYMERASE PB2 SUBUNIT IN COMPLEX WITH HUMAN IMPORTIN ALPHA5

ENGINEERED RESIDUE IN CHAIN A, ARG 389 TO LYS ENGINEERED RESIDUE IN CHAIN B, ARG 389 TO LYS ENGINEERED RESIDUE IN CHAIN D, ARG 389 TO LYS ENGINEERED RESIDUE IN CHAIN E, ARG 389 TO LYS ENGINEERED RESIDUE IN CHAIN F, ARG 389 TO LYS

7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE (MGT):
CO-CRYSTALLISED
SELENOMETHIONINE (MSE): SELENOMETHIONATED FOR STRUCTURE
DETERMINATION

MUTATION R389K IN OUR SEQUENCE COMPARED TO DATABASE.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

RNA-DEPENDENT RNA POLYMERASE, TRANSCRIPTION, PB2 SUBUNIT, INFLUENZA VIRUS, CAP-BINDING DOMAIN