Crystal structure of the stem and receptor binding domain of the spike protein P1 from bacteriophage PM2

Insights Into Virus Evolution and Membrane Biogenesis from the Structure of the Marine Lipid-Containing Bacteriophage Pm2.

CLONED FRAGMENT STARTS AT RESIDUE 82 AND STOPS AT RESIDUE 335
STEM-RECEPTOR BINDING DOMAIN, RESIDUES 82-335

THE ELECTRON DENSITY MAP FOR THE STEM DOMAIN WAS GREATLY IMPROVED BY USING BUSTER PROGRAM IN THE EARLY REFINEMENT CYCLES

7.3MG/ML (IN 150MM NACL, 20MM TRIS PH 7.5, 10MM CACL2),20%(W/V) PEG6K,100MM TRIS PH 8, 200MM LICL

CRYSTAL STRUCTURE OF THE RECEPTOR BINDING DOMAIN OF THE SPIKE PROTEIN P1 FROM BACTERIOPHAGE PM2

X-RAY STRUCTURE OF THE ENTIRE LIPID- CONTAINING BACTERIOPHAGE PM2

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DESPITE HAVING TWO MOLECULES IN THE ASU THE RESPECTIVE STEM DOMAINS FLEX WITH DIFFERENT ANGLES RELATIVE TO THE RECEPTOR DOMAIN. THEREFORE NO STRICT NCS

VIRAL STEM-RECEPTOR BINDING DOMAIN, VIRAL PROTEIN