Structure of human PYCR1 complexed with L-tetrahydro-2-furoic acid

In crystallo screening for proline analog inhibitors of the proline cycle enzyme PYCR1.

Pyrroline-5-carboxylate reductase 1, mitochondrial

Crystallization reservoir contained 3 M NaCl and 0.1 M HEPES at pH 7.5-8.0. Crystal was soaked in cryobuffer containing 50 mM L-tetrahydro-2-furoic acid and 30% glycerol.

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Pyrroline-5-carboxylate reductase 1, mitochondrial (E.C.1.5.1.2)

AMINO-ACID BIOSYNTHESIS, OXIDOREDUCTASE, PROLINE BIOSYNTHESIS